Scientific Management Theory Analysis

Scientific Management Theory Analysis The emphasis on increasing productivity from individual worker impels the emergence of F. W. Taylors scientific management at the beginning of 20th century. His philosophy of rationalizing work and organization to achieve maximum productivity, cooperation and prosperity has influenced the production and management model of his age profoundly. Despite the past 100 years, the shadow of Taylors philosophy in terms of production approach and management practice is still alive at the times of information technology, flexibility of production and the industrial restructuring. As Stern has written, The scientific management of Fredrick Taylor shaped the first coherent school of thought with its twin goal of productivity and efficiency- still influences management thinking 100 years on. This essay will assess the influence of scientific management on contemporary organization. Before assessing the influence, it is also important to introduce what scientific management is. It is a set of systematic theory of the correlation between labour and tasks for the purpose of increasing productivity by redesigning the work process. Based on the famous time-motion-study, Taylor developed four principles to increase efficiency: Study the way workers perform their tasks, develop a science for each element of a mans work, which replaces the old rule- of-thumb method. Codify the new methods of performing tasks into written rules and standard operating procedures Carefully select workers who posses skills and abilities that match the needs of the task, and train them to perform the task according to the established rules and procedures Establish a fair or acceptable level of performance for a task, and then develop a pay system that provide a reward for performance above the acceptable level Scientific management is a complete and interrelated system, but this essay will examine Taylors four principle separately to simply the analysis. One best way and division of labour Generally, Taylor believes that there is a one best way of each task to achieve maximum productivity and during his age, He firmly believes the division of labour is the best way to achieve this. Though nowadays, due to the diversification of the market and the organization, the simple one best way can hardly be defined, such as business adopt mass-production strategy can have a completely different optimal method of production than those focus on niche marketing, his spirit of pursuing the best way is still vivid presented. Just as the quality circle pioneered by Japanese car manufacturing, modification is being discussed continuously to make the work process more streamlined, more efficient and not wasteful in its activity, which is essentially the goal of Taylors system.(Stoney, 2001:p27) Especially when information technology has become the means of analyzing the underlying flow of material and information, it is more convenient and prevalent for contemporary organization to deci de the best redesign of business process to pursuing productivity, quality and competitive posture. Incorporated in his spirit, division of labour is the specific outcome of scientific management, the best way Taylor advocates to minimize skills required, and assembly-line, the creation of his age, are also still utilized successfully in contemporary fast-food industry. Such as MacDonald, the unskilled tasks from broken-down cooking procedure and sophisticated time record make it a completely modern duplication of Taylorism. The assembly lined production mode underpins the repetitive work cycle on the line by setting standard times. By doing this, efficiency improves dramatically as unnecessary tasks are eliminated, physical layouts improved, and work speeded up. (Fincham Rhodes, 2005: p678) Despite that deskilling () will de-motivate employees significantly on a general scale, which may result in absenteeism and high staff turnover, its ability of integrating new workers in production processes and dismissing workers without losing knowledge form the organization can successfully overcome it. Besides, the emphasis on quantity rather than quality (Ritzer, 2004) of the fast food industry also highlights the need of efficiency. Taylors influence goes beyond the bounds of manufacturing, the growing army of clerks in the rising service sector are automatically divided into departments and specialized in function. (Fincham Rhodes, 2005: p608) This means the philosophy of division of labour apply to most clerical works to rationalize the working procedure. A merging characteristics of clerical and production labour () becomes one remarkable feature of modern society. However, the anomie and alienation brought by the absolute division of labour limit its further application. The knock-on effect of de-motivate employees on quality and service can have more significant negative impact on contemporary organization than ever when they become the key to profitability at modern times. Despite the limitation of his method, this principle has a considerable profound and lasting influence because of Taylors preoccupation with the efficient use of resources. This philosophy can almost apply to every organization because whichever the work structure is taken, such as the prevalent team work and job enrichment; one of its ultimate goals should be improve efficiency. It can be proved by xxs argument that ()quality circles, rather than being a transformation of labor relations toward democratic participation, are managerial strategy to facilitate workers cooperation with managements goals for efficiency and productivity improvements. Standardization and direct control Taylor proposes standard rules to capture the best practice and direct control of workers to maintain efficiency. As Braverman (1974, p. 47) recognised, Taylorism never was a science, but a control system. Due to separate conception and execution, managers should design, allocate tasks and supervise workers performance without consulting employees opinions.(McGeorge Thoery X) Actually the appliance of Taylors efficiency through rationalization (Stoney, 2001: p.27) builds a mechanistic organizations, with strict rules, standardized defined tasks and top-down communication. Contemporarily, there are still cases where mechanistic organization proves to be optimal. A classic example is call centre where standard script must be followed, number of calls per hour is strictly monitored, as well as managers can listen in calls momentarily. Though it is argued that this complete control will alienate employees as it erodes the sense of freedom and self-determination, only this mechanic struct ure, where all tasks are designed to be consistent and coordinated, rigid rules are followed and instructions are obeyed immediately, can achieve the speedy service and uniform quality. Besides, despite its formalized and hierarchy nature causes inflexibility, organizations, such as call centre, which exist in a relatively stable environment where not much innovation and adjustment are required, has proved to be more effective because of the systematically rationalize procedures. (Burns Stalker, 1961) Thus, it still exists where efficiency dominates effectiveness. More commonly, only element of direct control can be seen in most contemporary organization. For instance, in public accounting firms, supervisor accountants supervise lower-rank accountants assigned with them to each audit. Junior accountants should work under the direct guidance of a senior accountant.(Hall, 1968) This is still due to Taylors inherent advantage, direct control can boost efficiency by rationalizing complex accounting procedure and adopting tasks efficiently and accurately. A further reason is that when meticulousness it the first requirement and errors may cause serious consequence, it is direct control that can maintain efficiency at the same time safeguard the quality. Efficient as it is, the reasons for not being fully adopted is that the drawbacks of direct control largely limit its influence. As Friedman (1977) points out, it neglects the positive aspects of labour. That means know-how and practical experience cannot be reflected and diffused. And the formalized system develops passive organization culture which undermines innovation, commitment and flexibility. This contradicts to the requirement of contemporary organization, especially for manufacturing. At modern times, in manufacturing sphere, the key to profitability lies in innovation.(Ackroyd, 2002) It means how to improve application for existing technology, how to create market advantage by good design and exclusivity become the very crucial issues. This is why most of contemporary organizations only retain elements of direct control and tend to be decentralized to emphases on democratic participation. To a broader extent, Taylors standardization philosophy is more widely used, and the new globalized era makes it an urgent requirement for business. Due to the great advantage of diffuse best practice, standardization becomes the key to facilitate the diffusion of businesss know-how to a new area for achieving a competitive advantage. Meanwhile, it is necessary for coordinating activities on a world wide scale and maintaining strict quality to safeguard firms reputation. However, exposed to a greater level of external complexity, the drawback of rigidity brought by standardization must be overcome, thus standardization is a continuous improvement process nowadays, just as the standard operation in Japanese car manufacturing where work is performed according to standard work sequence to insure quality, meanwhile standard if frequently updated to standardize improvement.(Edwards et al, 1993) To achieve this, standardization tends to be combined with a flatter and organic organization s tructure highlighting integration and decentralization to keep innovative and flexible. Scientific selection and training This is perhaps the most relevant principle today because these processes, selection, performance, as well as appraisal and development have been highly formalized in many organizations today (Cole, 2004). In the knowledge-based economy, talent has become the very valuable asset of business to achieve competitiveness. Much effort is devoted by contemporary organizations on selecting the right person. The contribution of Taylor is that he introduced the importance of selection criteria by management, such as the common competencies (i.e. communication skill, initiative) listed by recruitment department. In line with his emphasis on scientific approach to selection, Taylor advocates scientific training as he argues that it is only when business systematically cooperating to train the competent manà ¢Ã¢â€š ¬Ã‚ ¦that it shall be on the road to national efficiency. (Taylor, 1911: p 98)In the context of the knowledge society, companies are generally encouraged to develop employees skills and knowledge (Hansson, 2007) Such as Nissan UK believes the key way for being the most productive car plant in Europe is its ambitious training scheme involving its entire manufacturing workforce.(Lydon, 2007) Costly as it seems, the long-term benefits of quality, safety performance and manufacturing costs overweight it. Moreover, training is usually positively related to promotion (Bayo-Moriones OrtÄ ±Ãƒâ€šÃ‚ ´n-Angel 2006) it means it can motivate employees by satisfying their growth, achievement needs. Overstepping Taylors efficiency idea, nowadays the meaning of training is also related to be flexible and acuity enough for competing in a more complex global market, such as the widely-used management trainee schemes in multinationals to cultivate managers with practical experience and global perspective. Money as a motivator Taylor advocates piece-rate payment scheme based on his assumption that human nature is essentially self-interest, workers would be motivated by obtaining the highest possible wage by working in the most efficient and productive way. (Mullins, 2001: p23) In modern age, despite his method can rarely be seen due to the decadence of manufacturing, Based on his philosophy, money motivation still play a crucial role in other form, such as the incentive-based pay system. One common example is sales bonus. The rush of a sales force to place orders before month end reflects highly targeted performance. Thus there is little doubt that this system will work if designed appropriately. In the new context, when various approaches (i.e. job enrichment, team work) are applied to improve personal commitment, money motivator is not narrowed for efficiency as well. For instance, bonuses assessed on cooperation can represent up to 50% of wage packet in Japanese system and group bonuses are also given. (cf. Dohse et al., 1985, pp. 137-8) It means money motivator is now used flexibly according to organizations strategy. Ryness study of pay motivation in contemporary organization also support Taylors assumption, as there is overwhelming evidence that money is an important motivator for most people. Furthermore, he found that for high academic achievers, high performing employees and individuals with high self-efficiency and high needs for achievement, pay are the most important motivator.(Rynes et al, 2004) It means nowadays pay maybe more crucial than ever as those kinds of person are just the valuable human resources that organizations compete for. However, it is undoubted that Taylors idea of motivation is narrow minded and not suit as an extrinsic motivator, the emphasis on money will decrease staffs intrinsic motivator. Despite that, contemporary organizations usually adopt compromising approach as nowadays, the satisfaction of social needs and achievement needs are regarded as almost equality important motivator. Thus, multiple motivators, money in conjunction with other intrinsic motivator are more prevalent. For instance, performance-based pay and challenging work are used in such successful firms as Microsoft. Conclusion remark It should be admitted that there is almost no other management theory can overstep the influence of scientific management. As Braverman says, the principle of scientific management is not a failed system, but a set of guiding principles which continue to inform and influence the role and function of modern management. Some of the methods he advocates, such as division of labour, scientific selection and training, have become the features of modern society. More primarily, as efficiency is one of the enduring needs of all organizations, his preoccupation with the efficient use of resources thus becomes the driving force behind the evolution of subsequent management theories () and the root of management practice. Due to its extensive and deep influence, it is institutionalized not only in contemporary corporation, but also ideologically embraced in other institutions, modelling the modern world with the character of efficiency, calculability, predictability and control through technol ogy. (Ritzer) However, its inherent drawbacks of inflexibility, dehumanization require modification in current situation. Therefore, it can be argued that management of contemporary organization is based on a modification of Taylorism and a combination with more sophisticated management theories.

Puerto Ricans Immigrating to America Essay -- Puerto Rican History Cul

Puerto Ricans Immigrating to America The migration of Puerto Ricans to the United States occurred in two major waves. The first wave was in the 1910s-1940s and the second wave was from the 1960s to the 1990s. Each wave of migrants brought new generations of Puerto Ricans to the United States. Both waves of migrants believed that they were going to live a better life in America and migrated to major cities such as New York City, Chicago, Hartford, etc. The early migrants looked for industrial jobs such as in cigar factories while the later migrants found agricultural work such as in tobacco fields. The communities in which they lived grew larger and larger due to chain migration and because of this, the need for politics evolved. However, the type of politics that evolved as a result were different for each wave, yet had many similarities as well. If you would like to learn a little more about Puerto Rican migration itself, read Jamie Hellman's paper and Shakira Ramos' which goes into more depth. Class politics was the major form of politics that evolved during the early half of the twentieth century. According to Bernardo Vega, the tabaqueros were the only organized group in existence. They formed many clubs whose membership was not limited to Puerto Ricans only. Although the clubs consisted mainly of Cuban and Puerto Ricans, other people of different ethnic origins were members. One reason for this can be that the main leaders were exiles who had radical views and wanted change quick. Their concern was of homeland issues and they fought for independence from Spain. Concentrating on home issues, they paid no attention to their hostland (meaning America) issues because they lived with the belief that they were... ...from The Commuter Nation: Perspectives on Puerto Rican Migration," ed. By Carlos Antonio Torre, Hugo Rodriguez Vecchini, and William Burgos. (Rio Piedras, PR: Editorial de la Universidad de Puerto Rico, 1994), 313-328 2. James Dietz, Migration and International Corporations: The Puerto Rican Model of Development," ed. By Carlos Antonio Torre, Hugo Rodriguez Vecchini, and william Burgos. (Rio Piedras, PR: Editorial de la Universidad de Puerto Rico, 1994), 153-170 3. Tom Seidl, Janet Shenk, and Adrian DeWind, "The San Juan Shuttle: Puerto Ricans on Contract," from The Puerto Ricans: Their History, Culture, and Society, ed. by Adalberto Lopez. 2d. Ed. (New York: Shenkman, 1980), 417-431 . Video: "Pa’lante, Siempre Pa'lante!" [The Story of the Young Lords Party], Written and Directed by Iris Morales.

Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas Putida

Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida A Project done under the guidance of Dr. K. Bharathi Department of Biotechnology. Submitted to the faculty Of Department of Biotechnology National Institute Of Technology, Warangal (A. P) Submitted By Febin P. Nalpady, Anzal Rahman, Shruti Sharma, Sindhuja Nandiraju, Giraboina Kranthi Kumar NATIONAL INSTITUTE OF TECHNOLOGY WARANGAL (A. P) (DEEMED UNIVERSITY) 2010-2011 DEPARTMENT OF BIOTECHNOLOGY NATIONAL INSTITUTE OF TECHNOLOGY, WARANGAL (A. P) CERTIFICATE This is to certify that the project entitled â€Å"†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. † carried out by †¦.. , bearing roll no. †¦. ,, final year B. Tech, Biotechnology, during academic year 2010-2011, is a bonafide work submitted to the National Institute of Technology, Warangal in partial fulfillment of the requirements for the requirements for the award of the Degree of Bachelor of Technology. Guide : Dr. K Bharathi Dept. of Biotechnology NIT Warangal ACKNOWLEDGEMENT With great pleasure and deep sense of gratitude, we take this opportunity to express our sense of indebtedness to Dr. K Bharathi, our project guide for accepting us under her good self to carry out this project work, and providing us his invaluable guidance and constant encouragement at each and every step throughout the progress of this project. To be sincere it was an inextinguishable treasure of pleasure for us to work under her excellent guidance. I would also like to thank the faculty of our department,Dr. P Sreenivasa Rao, Mr. Onkara Perumal and Mr. K. Narasimhulu. They were a pillar of strength for us and encouraged us to do our best. Name Roll no Table of Contents 1. Abstract 2. Introduction 2. 1 Bioremediation 2. 2 The conventional techniques of remediation 2. 3 Advantages of Bioremediation 2. 4 Microbes that are useful for bioremediation 3. Review of literature 3. 1 Microbial degradation 3. 2 Biodegradation of petroleum hydrocarbons 3. 3 Factors affecting Degradation 3. 4 Mechanism of Petroleum Hydrocarbon Degradation 4. Brief outline of the project work 5. Materials and Methods 5. 1 Preparation Of Nutrient Broth 5. 2 Preparation of Nutrient Agar Slants from the Bacterial Strain 5. Preparation of SubCultures of Bacterial Strain 5. 4 Centrifugation of Crude Oil 5. 5 Subculturing Of Petri Plates with oil 5. 6 Biodegradation Studies 5. 7 Gravimetric Analysis 6. Results 6. 1 Growth Analysis of Pseudomonas Putida 6. 2 Gravimetric Analysis 7. Discussion 8. References 1. ABSTRACT Oil spills have become a serious problem with the ever-increasing re source exploitation, transportation, storage, and accidental leakage of oil. Several techniques, including physical, chemical, and biological methods, are used to recover spilled oil from the environment. Bioremediation is a promising option for remediation since it is effective and economic in removing oil with less undue environmental damages. However, it is a relatively slow process and the degree of success depends on a number of factors. These factors include the existence of a microbial population capable of degrading the pollutants, the availability of contaminants to the microbial population and the environment factors are type of soil, temperature, pH, the presence of oxygen and nutrients. This project aims to study the degradation extent of the pseudomonas putida on oil. The microbial strain used is procured from NCL pune. 2. Introduction In quantitative terms, crude oil is one of the most important organic pollutants in marine environment and it has been estimated that worldwide somewhere between 1. 7- 8. 8? 106 tons of petroleum hydrocarbons impact marine waters and estuaries annually. Reports have been appearing since last three decades on the biodegradability of crude oil by bacteria which can use hydrocarbons as source of carbon and energy. A way to mitigate the effects of oil spills is bioremediation. 2. 1 Bioremediation It is a process by which chemical substances are degraded by bacteria and other microorganisms. The use of these microorganisms has been successfully applied for the treatment of waste and wastewater in controlled systems. Several research studies have recently been performed to investigate the use of bioremediation for oil-spill cleanup in seawater, freshwater and terrestrial areas. The technique has been found to have a potential for broad applications in terrestrial and freshwater environments for treating soils and sediments contaminated with oil and other substances, as well as for coastal environments impacted by oil spills. Water is a more sensitive medium than soil and requires different remediation techniques. Spills to surface water are easier to clean up than spills to groundwater, for obvious reasons. It is not only much harder to see the extent of the contamination, but also to remove the source of the contamination as, for example, a leaking underground storage tank. 2. 2. The conventional techniques of remediation . The conventional techniques used for remediation have been to dig up contaminated soil and remove it to a landfill, or to cap and contain the contaminated areas of a site. The methods have some drawbacks. The first method simply moves the contamination elsewhere and may create significant risks in the excavation, handling, and transport of hazardous material. Additionally, it is very difficult and increasingly expensive to find new landfill sites for the final disposal of the material. A better approach than these traditional methods is to completely destroy the pollutants if possible, or at least to transform them to innocuous substances. Some technologies that have been used are high-temperature incineration and various types of chemical decomposition (e. g. , base-catalyzed dechlorination, UV oxidation). They can be very effective at reducing levels of a range of contaminants, but have several drawbacks, principally their technological complexity, the cost for small-scale application, and the lack of public acceptance, especially for incineration that may increase the exposure to contaminants for both the workers at the site and nearby residents. . 3 Advantages of Bioremediation Bioremediation is an option that offers the possibility to destroy or render harmless various contaminants using natural biological activity. As such, it uses relatively low-cost, low-technology techniques and can often be carried out on site. It will not always be suitable, however, as the range of contaminants on which it is effective is limited, the timescales involved are relatively long, and the residual contaminant levels achievable ma y not always be appropriate. Although the methodologies employed are not technically complex, considerable experience and expertise may be required to design and implement a successful bioremediation program, due to the need to thoroughly assess a site for suitability and to optimize conditions to achieve a satisfactory result. Because bioremediation seems to be a good alternative to conventional clean-up technologies research in this field, especially in the United States, rapidly increasing. Bioremediation has been used at a number of sites worldwide, including Europe, with varying degrees of success. Techniques are improving as greater knowledge and experience are gained, and there is no doubt that bioremediation has great potential for dealing with certain types of site contamination. Unfortunately, the principles, techniques, advantages, and disadvantages of bioremediation are not widely known or understood, especially among those who will have to deal directly with bioremediation proposals, such as site owners and regulators. 2. 4 Microbes that are useful for bioremediation The biodegradation of petroleum in the marine environment is carried out largely by diverse bacterial populations, including various Pseudomonas species. The hydrocarbon-biodegrading populations are widely distributed in the world’s oceans; surveys of marine bacteria indicate that hydrocarbon-degrading microorganisms are ubiquitously distributed in the marine environment. Generally, in pristine environments, the hydrocarbon-degrading bacteria comprise < 1% of the total bacterial population. These bacteria presumably utilize hydrocarbons that are naturally produced by plants, algae, and other living organisms. They also utilize other substrates, such as carbohydrates and proteins. When an nvironment is contaminated with petroleum, the proportion of hydrocarbon-degrading microorganisms increases rapidly. In particular, in marine environments contaminated with hydrocarbons, there is an increase in the proportion of bacterial populations with plasmids containing genes for hydrocarbon utilization. The proportion of hydrocarbon-degrading bacterial populations in hydrocarbon-contaminated marine environments often exceed 10% of the tot al bacterial population 3. Review of Literature 3. 1 Biodegradation of petroleum hydrocarbons Biodegradation of petroleum hydrocarbons is a complex process that depends on the nature and on the amount of the hydrocarbons present. Petroleum hydrocarbons can be divided into four classes: the saturates, the aromatics, the asphaltenes (phenols, fatty acids, ketones, esters, and porphyrins), and the resins (pyridines, quinolines, carbazoles, sulfoxides, and amides) [R. R. Colwell, J. D. Walker, and J. J. Cooney, â€Å"Ecological aspects of microbial degradation of petroleum in the marine environment,]. Di? erent factors in? uencing hydrocarbon degradation have been reported by Cooney et al. One of the important factors that limit biodegradation of oil pollutants in the environment is their limited availability to microorganisms. Petroleum hydrocarbon compounds bind to soil components, and they are difficult to be removed or degraded [S. Barathi and N. Vasudevan], â€Å"Utilization of petroleum hydrocarbons by Pseudomonas ? uorescens isolated from a petroleum-contaminated soil]. Hydrocarbons di? er in their susceptibility to microbial attack. The susceptibility of hydrocarbons to microbial degradation can be generally ranked as follows: linear alkanes > branched alkanes > small aromatics > cyclic alkanes [J. J. Perry, â€Å"Microbial metabolism of cyclic alkanes,† in Petroleum Microbiology]. Some compounds, such as the high molecular weight polycyclic aromatic hydrocarbons (PAHs), may not be degraded at all. 3. 2 Microbial degradation Microbial degradation is the major and ultimate natural mechanism by which one can cleanup the petroleum hydrocarbon pollutants from the environment [1-3] The recognition of biodegraded petroleum-derived aromatic hydrocarbons in marine sediments was reported by[ Jones et al]. They studied the extensive biodegradation o alkyl aromatics in marine sediments which occurred prior to detectable biodegradation of n-alkane pro? e of the crude oil and the microorganisms, namely, Arthrobacter, Burkholderia, Mycobacterium, Pseudomonas, Sphingomonas, and Rhodococcus were found to be involved for alkylaromatic degradation. Microbial degradation of petroleum hydrocarbons in a polluted tropical stream in Lagos, Nigeria was reported by Adebusoye et al. Nine bacterial s trains, namely, Pseudomonas ? uorescens, P. aeruginosa, Bacillus subtilis, Bacillus sp. , Alcaligenes sp. , Acinetobacter lwo? ,Flavobacteriumsp. , Micrococcus roseus, and Corynebacterium sp. were isolated from the polluted stream which could degrade crude oil. Hydrocarbons in the environment are biodegraded primarily by bacteria, yeast, and fungi. The reported e? ciency of biodegradation ranged from 6% to 82% for soil fungi, 0. 13% to 50% for soil bacteria, and 0. 003% to 100% [6] for marine bacteria. Many scientists reported that mixed populations with overall broad enzymatic capacities are required to degrade complex mixtures of hydrocarbons such as crude oil in soil, fresh water, and marine environments [8]. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment [7]. Several bacteria are even known to feed exclusively on hydrocarbons [9]. Floodgate [36] listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated from marine environment. A similar compilation by Bartha and Bossert [6] included 22 genera of bacteria and 31 genera of fungi. In earlier days, the extent to which bacteria, yeast, and ? lamentous fungi participate in the biodegradation of petroleum hydrocarbons was the subject of limited study, but appeared to be a function of the ecosystem and local environmental conditions [7]. Crude petroleum oil from petroleum contaminated soil from North East India was reported by Das and Mukherjee . Acinetobacter sp. Was found to be capable of utilizing n-alkanes of chain length C10–C40 as a sole source of carbon [6]. Bacterial genera, namely, Gordonia, Brevibacterium, Aeromicrobium, Dietzia, Burkholderia, and Mycobacterium isolated from petroleum contaminated soil proved to be the potential organisms for hydrocarbon degradation [9]. The degradation of poly- aromatic hydrocarbons by Sphingomonas was reported by Daugulis and McCracken . Fungal genera, namely, Amorphoteca, Neosartorya, Talaromyces, and Graphium and yeast genera, namely, Candida, Yarrowia, and Pichia were isolated from petroleum contaminated soil and proved to be the potential organisms for hydrocarbon degradation [ Singh et al. ] also reported a group of terrestrial fungi, namely, Aspergillus, Cephalosporium, and Pencillium which were also found to be the potential degrader of crude oil hydrocarbons. The yeast species, namely, Candida lipolytica, Rhodotorula mucilaginosa, Geotrichum sp, and Trichosporon mucoides isolated from contaminated water were noted to degrade petroleum compounds [5]. Though algae and protozoa are the important members of the microbial community in both aquatic and terrestrial ecosystems, reports are scanty regarding their involvement in hydrocarbon biodegradation. [Walker et al. ] isolated an alga, Prototheca zop? which was capable of utilizing crudeoil and a mixed hydrocarbon substrate and exhibited extensive degradation of n-alkanes and isoalkanes as well a aromatic hydrocarbons. Cerniglia et al. observed tha nine cyanobacteria, ? ve green algae, one red alga, one brown alga, and two diatoms could oxidize naphthalene. Protozoa by contrast, had not been shown to utilize hydrocarbons. 3. 3 Factors affecting Degradation A number of limiting factors have been recognized to a? ect the biodegradation of petroleum hydrocarbons, many of which have been discussed by Brusseau. The composition and inherent biodegradability of the petroleum hydrocarbon pollutant is the ? rst and foremost important consideration when the suitability of a remediation approach is to be assessed. Among physical factors, temperature plays an important role in biodegradation of hydrocarbons by directly a? ecting the chemistry of the pollutants as well as a? cting the physiology and diversity of the microbial ? ora. Atlas [4] found that at low temperatures, the viscosity of the oil increased, while the volatility of the toxic low molecular weight hydrocarbons were reduced, delaying the onset of biodegradation. Temperature also a? ects the solubility of hydrocarbons [8]. Although hydrocarbon biodegradation can occur over a wide range of temperatures, the rate of biodegradation generally decreases with the decreasing temperature. shows that highest degradation rates that generally occur in the range 30–40? C in soil environments, 20–30? Cin some freshwater environments and 15–20? C in marine environments . Venosa and Zhu [11] reported thatambient temperature of the environment a? ected both the properties of spilled oil and the activity of the microorganisms. Signi? cant biodegradation of hydrocarbons have been reported in psychrophilic environments in temperate regions. Nutrients are very important ingredients for successful biodegradation of hydrocarbon pollutants especially nitrogen, phosphorus, and in some cases iron [8]. Some of these nutrients could become limiting factor thus a? ecting the biodegradation processes. Atlas [11] reported that when a major oil spill occurred in marine and freshwater environments, the supply of carbon was signi? cantly increased and the availability of nitrogen and phosphorus generally became the limiting factor for oil degradation. In marine environments, it was found to be more pronounced due to low levels of nitrogen and phosphorous in seawater [10]. Freshwater wetlands are typically considered to be nutrient de? cient due to heavy demands of nutrients by the plants. Therefore, additions of nutrients were necessary to enhance the biodegradation of oil pollutant. On the other hand, excessive nutrient concentrations can also inhibit the biodegradation activity [11]. Several authors have reported the negative e? ects of high NPK levels on the biodegradation of hydrocarbons especially on aromatics [10]. The e? ectiveness of fertilizers for the crude oil bioremediation in subarctic intertidal sediments was studied by Pelletier et al. . Use of poultry manure as organic fertilizer in contaminated soil was also reported , and biodegradation was found to be enhanced in the presence of poultry manure alone. Maki et al. eported that photo-oxidation increased the biodegradability of petroleum hydrocarbon by increasing its bioavailability and thus enhancing microbial activities. 3. 4 Mechanism of Petroleum Hydrocarbon Degradation The most rapid and complete degradation of the majority of organic pollutants is brought about under aerobic conditions. Figure 2 shows the main principle of aerobic degradation of hydrocarbons [11]. The initial intracellular at tack of organic pollutants is an oxidative process and the activation as well as incorporation of oxygen is the enzymatic key reaction catalyzed by oxygenases and peroxidases. Peripheral degradation pathways convert organic pollutants step by step into intermediates of the central intermediary metabolism, for example, the tricarboxylic acid cycle. Biosynthesis of cell biomass occurs from the central precursor metabolites, for example, acetyl-CoA, succinate, pyruvate. Sugars required for various biosyntheses and growth are synthesized by gluconeogenesis. The degradation of petroleum hydrocarbons can be mediated by speci? c enzyme system. Figure 3 shows the initial attack on xenobiotics by oxygenases. Other mechanisms involved are (1) attachment of microbial cells to the substrates and (2) production of biosurfactants [12]. The uptake mechanism linked to the attachment of cell to oil droplet is still unknown but production of biosurfactants has been well studied. 4. Brief outline of the project work: 1. Procurement of oil Samples. 2. Procurement of Pseudomonas putida strain. 3. Sub-culturing the microbe in nutrient rich media for checking viability.. 4. Culturing microbes on a mineral salt media containing only crude oil as a carbon source. 5. Biodegradation studies. 6. Gravimetric analysis 5. Materials and Methods Soil Samples – Samples(500g) contaminated with oil used for hydrocarbons utilizing microorganisms, were collected from Nhava Sheva port in Mumbai(where a recent oil spill has took place). Crude Oil – Crude Oil is procured from an Oil production site of ONGC. Bacterial Strain – Pseudomonas Putida PS-I strain procured from NCL Pune. 5. 1 Preparation Of Nutrient Broth For preparation of nutrient agar, malt extract, yeast extract, Potassium dihydrogen phosphate and dextrose is required. Malt extract and yeast extract is generally used as a nutritious agent. Potassium dihydrogen phosphate i. . KH2PO4 is used as a buffering agent to maintain the pH. Dextrose is generally used as a carbon source because dextrose inhibits the growth of other micro-organisms. AUTOCLAVE is a device to sterilize equipment and supplies by subjecting them to high pressure steam at 121 ° C or more. Machines in this category largely operate by utilizing pressurized steam and superheated water. To sterilize culture media, rubber material, gowns, dressing, gloves etc. are used. It is particularly useful for materials which cannot withstand the higher temperature of hot air oven. CHEMICALS REQUIRED:- For 1000ml, Malt extract — 10 gm Beef Extract — 4 gm K2HPO4 — 1 gm Magnesium sulphate — 1 gm Sodium Chloride — 0. 5 gm pH — 7. 0 Agar — 15% PROCEDURE:- For preparation of 100ml of nutrient broth, around 100ml of double distilled water was taken in a conical flask. Malt extract, yeast extract, KH2PO4 and dextrose was weighed as per the composition mentioned above and added to the conical flask. The conical flasks are to be shaken so well so that all the chemicals should dissolve. pH was checked using pH meter and adjusted to 7. 0 using NaOH and HCl. The volume was made to 100ml by adding double distilled water. The above solution i. e. nutrient agar along with the Petri-plates was autoclaved at 15 psi and 15 minutes. Now the solution was allowed to cool down to ready to pour condition. PRECAUTIONS:- The autoclave should be done at 15 psi and 15 min. The pH should be maintained at 7. 0. 5. 2 Preparation of Nutrient Agar Slants from the Bacterial Strain For the preparation of Slants, Flame the inoculating loop to redness by holding it pointed down into the flame, starting near the handle and then moving the loop into the flame. This technique sterilizes the loop and, if wet with a culture, heats up the loop without spattering bacteria into the air and onto the surrounding area. Let the loop cool a minute. A hot loop will damage the bacteria cells. Using the fingers of the â€Å"loop hand† remove the cap from the stock culture tube and flame the tube mouth. Do not set the tube top down on the table. Insert the cooled sterilized loop into the culture tube being careful to not touch the sides of the tube. Touch the loop to the culture. You need not scrape a visible amount from the culture. Hold the tube as horizontal as possible to preclude particles from the air settling into the tube But do watch out for any condensate in the bottom of slant cultures. Don't let this fluid wash across the face of the culture. Remove the loop being careful again to not touch the tube sides. Flame the tube mouth and replace the cap. Remove the cap of the broth tube. Flame the top. Remember to hold the top in your fingers. Insert the loop into the Slant tube filled with agar and shake to remove the bacteria. Withdraw the loop, flame the tube mouth and replace the cap. Resterilize the inoculating loop and place it on the table. Never place a contaminated loop on the table. If there is any liquid in the bottom of the slant tube avoid sticking the loop into this condensate. 5. 3 Preparation of SubCultures of Bacterial Strain The Nutrient Broth Cultures are inoculated with the bacterial strain from the nutrient agar slant as detailed below. PROCEDURE Light your Bunsen burner. In one hand hold both the Nutrient Broth culture to be inoculated and the nutrient slant agar. Loosen the tube caps. In your other hand hold the inoculating loop. Flame the inoculating loop to redness by holding it pointed down into the flame, starting near the handle and then moving the loop into the flame. This technique sterilizes the loop and, if wet with a culture, heats up the loop without spattering bacteria into the air and onto the surrounding area. Let the loop cool a minute. A hot loop will damage the bacteria cells. Using the fingers of the â€Å"loop hand† remove the cap from the stock culture tube and flame the tube mouth. Do not set the tube top down on the table. Insert the cooled sterilized loop into the slant tube being careful to not touch the sides of the tube. Touch the loop to the culture. You need not scrape a visible amount from the culture. Hold the tube as horizontal as possible to preclude particles from the air settling into the tube But do watch out for any condensate in the bottom of slant cultures. Don't let this fluid wash across the face of the culture. Remove the loop being careful again to not touch the tube sides. Flame the tube mouth and replace the cap. Remove the cap of the broth tube. Flame the top. Remember to hold the top in your fingers. Insert the loop into the broth and shake to remove the bacteria. Gently shake the broth culture. This inoculated broth culture is incubated at room temperature for 72 hours and the bacteria is allowed to grow in the broth medium. 5. 4 Centrifugation of Crude Oil Centrifugation is a process that involves the use of the centrifugal force for the separation of mixtures with a centrifuge, used in industry and in laboratory settings. More-dense components of the mixture migrate away from the axis of the centrifuge, while less-dense components of the mixture migrate towards the axis. The precipitate (pellet) gathers on the bottom of the tube. The remaining solution is properly called the â€Å"supernate† or â€Å"supernatant liquid† The Crude Oil is Centrufuged at a speed of 5000 rpm for a period of ten minutes. The Contaminants in the oil are collected at the bottom of the tube in the form of pellets. These pellets can be removed by filtration using a filter paper. Now the concentrates oil which is free from impurities is collected in a flask and gently shaken. Spectophotometric Analysis Optical density, measured in a spectrophotometer, can be used as a measure of the concentration of bacteria in a suspension. As visible light passes through a cell suspension the light is scattered. Greater scatter indicates that more bacteria or other material is present. The amount of light scatter can be measured in a spectrophotometer. Typically, when working with a particular type of cell, you would determine the optical density at a particular wavelength that correlates with the different phases of bacterial growth. Generally we will want to use cells that are in their mid-log phase of growth. Typically the OD600 is measured. 5. 5 Subculturing Of Petri Plates with oil % of crude oil is mixed with 100 ml of Nutrient broth medium. The 1. 5g of agar is added to the medium and Nutrient Agar(with 1% crude oil) is prepared. Now take 6 Petri dishes. Open one of the dishes. Take the nutrient agar to be added and Swab the agar, barely pressing, side to side on the entire surface. The dish is closed immediately after swabbing to prevent contamination. The dish is sealed with tape around the edges to prevent co ntamination. Repeat the same procedure for the other dishes. Put the dishes in an incubator for 4 days to allow some growth. 5. 6 Biodegradation Studies Laboratory Biodegradation studies were carried out under optimized conditions for assessing the biodegradation potential of the pseudomonas putida PS-I Strain. After the desired interval of time, the petriplates were taken out and the bacterial activities were stopped by adding 1% N HCl. For the extraction of crude oil from these plates, 50ml of culture broth was mixed with 50 ml of acetone : petroleum ether (1:1) in a single separating funnel and shaken vigorously to get a single emulsified layer and acetone was added then to it and shaken gently to break the emulsification which resulted in three layers. Top layer was a mixture of Petroleum ether crude oil and acetone. Clumping cells aere formed in the middle layer and the bottom layer contains acetone, water and biosurfactant in soluble form. The lower two layers were separated out while the top layer containing petroleum ether mixed with crude oil and acetone is taken out in a fresh beaker. The extracted oil is passed through anhydrous sodium sulphate in order to remove the moisture. The petroleum ether and acetone were evaporated on a water bath leaving us with the dry oil clump. 5. 7 Gravimetric Analysis Gravimetric analysis describes a set of methods in analytical chemistry for the quantitative determination of an analyte based on the mass of a solid. the analyte must first be converted to a solid by precipitation with an appropriate reagent. The precipitate can then be collected by filtration, washed, dried to remove traces of moisture from the solution, and weighed. The amount of analyte in the original sample can then be calculated from the mass of the precipitate and its chemical composition. Gravimetric analysis is performed on the dry oil clump collected after the water bath. It is done by weighing the quantity of residual oil left after biodegradation in a tared vial. The mass of this crucible is subtracted from the initial mass of the 1% of oil that is added in the petridishes giving the amount of oil that is degraded due to the biological avtivity of the pseudomonas putida strain. 6. Results 6. 1 Growth Analysis of Pseudomonas Putida: The culture which was obtained in test tube slants was further sub cultured in conical flasks in a LB medium and the growth analysis was done to check the viability of the culture obtained. The growth kinetics plot was obtained by measuring the O. D. y using a visible spectrophotometer and recording the reading at regular intervals. The Graph was then plotted. 6. 2 Gravimetric Analysis: Biodegradation studies were conducted for 15 days and gravimetric analysis was done after every five days. The biodegradation effect was seen from the 5th day onwards. Laboratory biodegradation studies on crude oil by Pseudomonas putida No . Of Days| Initial Concn| Final Concn| Difference| Degradation (%)| 5 days| 1. 431  ± . 57| 1. 325  ± . 46| 0. 106  ± . 11| 7. 4| 10 days| 1. 453  ± . 71| 1. 198  ± . 38| 0. 255  ± . 34| 17. 54| 15 days| 1. 398  ± . 68| 0. 936  ± . 31| 0. 62  ± . 28| 33. 04 | 7. Discussion It can be seen that the degradation percentage of oil has increased from mere 7. 41 in the first 5 days to a good 33. 04 percentage towards the 15th day, from this it is clearly understood that pseudomonas putida is an ideal organism for bioremediation programmes. Moreover this rate of degradation has been obtained under normal conditions without any aid from surfactants or fertilizers. Hence there is scope for achieving much greater rates by using the above mentioned methods of fertilizing or adding surfactants. 8. BIBLIOGRAPHY (1). U. S. Enviromental Protection Agency (1990). Interim Report, Oil Spill Bioremediation Project. U. S. Environmental Protection Agency, Office of Research and Development, Washington (2). T. Cairney. Contaminated Land, p. 4, Blackie, London (1993). (3). R. B. King, G. M. Long, J. K. Sheldon. Practical Environmental Bioremediation: The Field Guide, 2nd ed. , Lewis, Boca Raton, FL (1997). (4). Atlas, Ronald M. (1995). Petroleum Biodegradation and Oil Spill Bioremediation. Marine Pollution Bulletin 31, 178-182 (5) Hoff, Rebecca Z. (1993). Bioremediation: an overview of its development and use for oil spill cleanup. Marine Pollution Bulletin 29, 476-481. 6). Irwin, Patricia (1996). To clean up environmental spill, know your medium. Electrical World 37-40. (7). Swannell, Richard P. J. ; Lee, Kenneth; McDonagh, Madeleine (1996). Field Evaluations of Marine Oil Spill Bioremediation. Microbiological Reviews 60, 342-365 (8). Radwan, S. S. ; Sorkhoh, N. A. ; El-Nemr, I. M. ; El-Desouky, A. F. (1997). A feasibility study on seeding as a bio remediation practice for the oily Kuwaiti desert. Journal of Applied Microbiology 83, 353-358. (9). P. E. Flathman, D. Jerger, J. E. Exner. Bioremediation: Field Experience, Lewis, Boca Raton, FL (1993). 10). J. G. Mueller, C. E. Cerniglia, P. H. Pritchard. Bioremediation of Environments Contaminated by Polycyclic Aromatic Hydrocarbons. In Bioremediation: Principles and Applications, pp. 125–194, Cambridge University Press, Cambridge (1996). (11). P. J. S. Colberg and L. Y. Young. Anaerobic Degradation of Nonhalogenated Homocyclic Aromatic Compounds Coupled with Nitrate, Iron, or Sulfate Reduction. In Microbial Transformation and Degradation of Toxic Organic Chemicals, pp. 307–330, Wiley-Liss, New York (1995). (12). A. S. Allard and A. H. Neilson. Oil Eating Microbes 39, 253–285 (1997).

“The Catcher in the Rye” Let’s Talk About Sex

When you proceed to the Chapter 9 of â€Å"The Catcher in the Rye†, you see Holden, who looks out of the window of his hotel and gazes at the rooms of the others, where his eyes are hooked by a couple of strangers, who squirt water into each other’s mouths and a â€Å"distinguished-looking† man in women’s outfit. Holden’s Mind-Blowing Questions What questions pop up in Holden’s head? The first one is why everyone he sees does not close the shades? And the following question that worries the reader – does the protagonist finds sex inherently degrading? It’s hard to provide an answer for the first question, but one can ponder over the question number 2. And the answer, in my personal opinion, is yes. When your heart beats faster when you’re seeing the girl you like, Holden believes, you act and think just like a â€Å"crumby stuff†. But the reader cannot be 100% sure about that. Moreover, at some point it seems that Holden cannot actually have sex with a young lady at all for the reason that it may turn the girl into an object. In the other words, Holden should either have sexual relationship with women he’s not in love with, or never have it at all. One more problem that Holden is faced with in his everyday life is that when he’s hanging out with a young lady and she actually asks to stop, Holden really†¦stops. For some unknown reason, the boy cannot reach the sense of balance between treating a girl with respect and taking sexual control of this or that situation where his girlfriend really desires him to. On the other hand, Holden has his own reasons to respect certain boundaries. The thing is that some time ago the boy has gone through a come-on at his teacher’s hands and perhaps Holden has experienced some â€Å"pervertion† for â€Å"about twenty times since he was a kid†. As for Jane, she either has or has not got physically abused by the stepfather (keep in mind that the main character makes use of â€Å"about twenty† very often in order to point out indeterminate (large) numbers). Where does this ambiguity come from? Well, a third person could probably give us an answer on what is going on in Holden’s head. But, unfortunately, we don’t have it now. All that we have while reading the book is Holden, who is a little bit sex-crazed and confused teen, who openly says he â€Å"doesn’t understand† sex. Can Holden Be Gay? This is the last question that worries a lot of readers of different ages. What if that is an actual reason why he is so alienated? Perhaps. Holden really spends a lot of days pondering over the hot bod of Stradlater. Maybe at some point he sees homosexuality where it actually does not exist, as with Mr. Antolini or Carl Luce. To say more, Holden doesn’t feel comfortable when thinking about intimacy with a female. And maybe that’s something that even Holden is afraid to admit to himself? This was an example of an essay on topic of â€Å"The Catcher in the Rye†, written by the writers of our custom writing service. To buy an essay on the necessary topic, please, fill in the Free Inquiry form in the top right corner of this page.